Electrophoresis Reagents | Nucleic Acid Electrophoresis Reagents | Protein Electrophoresis Reagents | Readymix gel and Chemicals | Buffer | Immuno Electrophoresis Reagents | India | Supplier | Exporter
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Tris (Tris (Hydroxymethyl)      Aminomethane)
Tris Buffer pH 8.8
Tris Buffer pH 6.8
Bis-Tris
Sodium Dodecyl Sulfate(SDS)
10% SDS
Tris-Glycine SDS buffer 10X     concentrate
Tris-Glycine buffer Native PAGE    10X
Tris-Tricine SDS buffer 10X
Tris-Borate EDTA buffer 5X
Western Blotting buffer 5X
Sample buffer for SDS-PAGE 5X
Sample buffer for Native PAGE 5X
Brilliant Blue G stain 2X
Brilliant Blue R stain 2X
Amido Black stain 2X
Ponceau's stain 2X
Destainer 4X
 
 
Tris (Tris (Hydroxymethyl) Aminomethane)

> Biotechnology Grade
> MW 121.14
> Purity: >99.8%
> Moisture: <1.0%
> Insolubles: <0.005%
> Solubility (10%, water): Clear and haze-free
> A280 (1.0M, water): <0.05
> As: <0.0005% Pb: <0.0001%
> Fe: <0.0001% Cu: <0.0001%
> Mg: <0.0001%
> DNase, RNase, Protease: None detected

Storage: Room Temperature
Ordering Information
Cat.No Product Units
BIO-0141-010 Tris-Base 100g
BIO-0141-500 Tris-Base 500g
BIO-0141-110 Tris-Base 1kg
BIO-0141-010 Tris-Base (Biotech Grade) 100g
BIO-0141-500 Tris-Base (Biotech Grade) 500g
BIO-0141-110 Tris-Base (Biotech Grade) 1kg
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Tris Buffer pH 8.8
Tris HCl 1.5M pH8.8 is a buffer useful for casting separating gel.
Storage: 4 ° C
Ordering Information
Cat.No Product Units
BIO-0142-010 Tris Buffer pH8.8 100ml
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Tris Buffer pH 6.8
Tris HCl 1.0M pH6.8 is a buffer useful for casting stacking gel.
Storage: 4 ° C
Ordering Information
Cat.No Product Units
BIO-0143-010 Tris Buffer pH6.8 100ml
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Bis-Tris
> Bis-(2-Hydroxymethyl)imino-tris(hydroxymethyl) Methane
> Ultra Pure Grade
> MW 209.2 Purity: >99.0%
> pKa ( 25 ° C): 6.50±0.2
> Melting range: 104 ± 2 ° C
Storage: Room Temperature
Ordering Information
Cat.No Product Units
BIO-0124-005 Bis-Tris 50g
BIO-0124-010 Bis-Tris 100g
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Sodium Dodecyl Sulfate(SDS)
> Biotechnology Grade
> MW 288.38
> Purity: >99.0%
> A260 (3%,water): <0.1 A280 (3%,water): <0.1
> Chloride: <0.1% Phosphate: <0.005%
> Pb: <0.005% Cu: <0.005%
> DNase, RNase: Not detected
Storage: Room Temperature
Ordering Information
Cat.No Product Units
BIO-0144-010 Sodium Dodecyl Sulphate (SDS) 100g
BIO-0144-500 Sodium Dodecyl Sulphate (SDS) 500g
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10% SDS
Sodium Dodecyl Sulphate 10g/100ml, the ionic detergent which binds strongly to the proteins.
Storage: Room Temperature
Ordering Information
Cat.No Product Units
BIO-0145-005 10% SDS 50 ml
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Tris-Glycine SDS buffer 10X concentrate
This buffer is used as tank buffer for SDS-PAGE. The buffer at 1X concentration contains 0.025M Tris, 0.192M glycine and 0.1%SDSpH 8.3-8.6.
Storage: 4 ° C
Ordering Information
Cat.No Product Units
BIO-0146-0110 Tris-Glycine SDS Buffer 10X Concentrate 1litre
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Tris-Glycine buffer Native PAGE 10X
This buffer is used as tank buffer for Native PAGE. The buffer at 1X concentration contains 0.025M Tris and 0.192M Glycine pH 8.3-8.6.
Storage: 4 ° C
Ordering Information
Cat.No Product Units
BIO-0147-0110 Tris-Glycine Buffer Native PAGE 10X 1litre
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Tris-Tricine SDS buffer 10X
This buffer is used as tank buffer for SDS-PAGE. The buffer at 1X concentration contains 0.1M Tris, 0.1M Tricine and 0.1% SDS pH 8.3-8.6.
Storage: 4 ° C
Ordering Information
Cat.No Product Units
BIO-0148-0250 Tris-Tricine SDS Buffer 10X 250 ml
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Tris-Borate EDTA buffer 5X
This buffer is used as Agarose Gel Electrophoresis buffer. The buffer at 1X concentration contains 0.02MTris, 0.1M Borate and 1mM EDTA pH7.6
Storage: 4 ° C
Ordering Information
Cat.No Product Units
BIO-0149-0250 Tris-Borate EDTA Buffer 5X 250 ml
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Western Blotting buffer 5X
This buffer is used as electro-transfer buffer for protein blotting from SDS-PAGE. The buffer at 1x concentration contains 15mM Tris and 120mM Glycine, 0.1% SDS and 20% Methanol pH 8.6.
Storage: 4 ° C
Ordering Information
Cat.No Product Units
BIO-0150-0500 Western Blotting Buffer 5X 500ml
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Sample buffer for SDS-PAGE 5X
Sample buffer at 5x concentration contains 4% SDS, 20% Glycerol, 10% b -mercaptoethanol, 0.004% bromophenol blue and 0.125M Tris-HCl, pH approximately 6.8. This is used for preparing sample for SDS-PAGE.
Storage: -20 ° C
Ordering Information
Cat.No Product Units
BIO-0151-0001 Sample Buffer for SDS PAGE 5X 5 x 2ml
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Sample buffer for Native PAGE 5X
Sample buffer at 5X concentration contains 20% Glycerol, 0.004% bromophenol blue and 0.125M Tris-HCl pH approximately 6.8. This is used for preparing sample for Native PAGE.
Storage: -20 ° C
Ordering Information
Cat.No Product Units
BIO-0152-0001 Sample Buffer for Native PAGE 5X 5 x 2 ml
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Brilliant Blue G stain 2X
Content of one bottle of brilliant blue G stain is to be made to a final volume of 1litre with deionized H 2 O. The final solution will contain 0.1% (w/v) brilliant blue G, 25% methanol and7%Acetic acid. This is used for staining protein in PAGE and Agarose gels.
Storage: Room Temperature
Ordering Information
Cat.No Product Units
BIO-0153-0500 Brilliant Blue G Stain 2X 500 ml
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Brilliant Blue R stain 2X
Content of one bottle of brilliant blue R stain is to be diluted to one litre with deionized H 2 O. The final solution contains 0.25% (w/v) brilliant blue R, 40% methanol and 7% Acetic acid. This is suitable for staining protein in polyacrylamide and agarose gels.
Storage: Room Temperature
Ordering Information
Cat.No Product Units
BIO-0154-0500 Brilliant Blue R Stain 2X 500 ml
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Amido Black stain 2X
After dilution to 1X concentration the solution will contain 0.1% (w/v) amido black, 25% (w/v) isopropanol and 10% Acetic acid. This is suitable for staining proteins on nitrocellulose membranes after transfer from SDS-PAGE visualizes proteins in microgram range with a clear background.
Storage: Room Temperature
Ordering Information
Cat.No Product Units
BIO-0155-0250 Amido Black Stain 2X 250 ml
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Ponceau's stain 2X
After dilution to 1X concentration the solution will contain 0.2% ponceau's, 3%TCA. This is suitable for staining proteins on nitrocellulose membranes after transfer from SDS-PAGE. Can visualize proteins in microgram range with a clear background.
Storage: Room Temperature
Ordering Information
Cat.No Product Units
BIO-0156-0250 Ponceau's Stain 2X 250 ml
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Destainer 4X
Content of one bottle of destainer is to be diluted 4 times with deionized water. The final solution contains 15% methanol and 7.5% Acetic acid. This is suitable for destaining polyacrylamide and agarose gels.
Storage: Room Temperature
Ordering Information
Cat.No Product Units
BIO-0157-0110 Destainer 4X 1 litre
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